The PRP-X400 column provides a fast separation for glyphosate and its metabolites. The exchange capacity of the PRP-X400 is greater than that of the PRP-X200, leading to characteristics better suited for the separation of glyphosate. It also performs well in other separations, such as inositol and sugar alcohols. The column does not have to be heated to 65°C and operates well at room temperature, so a column heater is not necessary for this method. PRP-X400 columns do not require the use of methanol in the mobile phase, and they cost much less than other glyphosate columns.