Starting in the mid 1970s Reversed Phase Liquid Chromatography (RPC) has become the standard technique to analyze small molecular weight compounds in industrial, academic and governmental laboratories.More recently, RPC has become the accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.Tosoh Bioscience offers 15 distinct Reversed Phase column types which are either based on silica or methacrylate particles as discussed below.
The silica-based TSKgel reversed phase column products consist of eleven stationary phases over
4 key product families. Each TSKgel silica column features:
The polymer-based TSKgel reversed phase columns are synthesized from polymethacrylate polymers in a range of pore and particle sizes. Advantages of polymethacrylate particles include:
High purity Type B Silica High efficiencies Excellent recoveries Low bleed for MS
Hydrophilic backbone to improve recovery
and reduce secondary interactions Stable from pH 2.0 to 12.0 Compatibility with organic solvents eliminates
swelling
High purity Type B Silica